Fig. 5.

Ethanol disrupts convergent extension. a Schematic of the in situ expression domains of shha (midline), pax2a (midbrain-hindbrain boundary), and dlx3 (anterior neural plate) at 10 hpf. The dotted line indicates the shha-dlx3 length measured in panel C. Dorsal view, anterior to the left. Created with BioRender.com. b Quantification for the normalized shha/pax2a expression domains (p < 0.05; Tukey’s honest test). Sample size and p values provided in Additional file 9: Table S2. c Quantification of the distance between shha and dlx3, as shown by the dotted line in panel A (p < 0.05; Tukey’s honest test). Sample size and p values provided in Additional file 9: Table S2. d Expression pattern of six3a via whole mount in situ hybridization, in control and ethanol-treated vangl2 embryos at 11 hpf