Fig. 2
From: Innate antiviral defense demonstrates high energetic efficiency in a bony fish
Response of sockeye salmon to IHNV infection. a Mean (line) and individual (dot) IHNV transcript loads and infection prevalence (bars) measured at 0, 1, 4, and 9 weeks post-challenge (wpc). b Kaplan-Meier survival curves of saline control (SC) and IHNV-injected (IHNV) fish (log-rank p < 0.001) ± SE. c Mean fold change (± SE) of interferon-stimulated gene (ISG) transcription measured as mx1, rsad2, and eif2ak2 mRNA transcripts relative to their mean expression in time-matched controls (SC); ***p < 0.001 by 2-way ANOVA with Dunnett’s multiple comparison tests using corrected normalized relative quantities (CNRQ); minimum twofold change suggestive of biological relevance is shaded. d Correlation of ISG transcript abundance relative to log-IHNV transcriptional load; Spearman r and correlative p-value provided; mean (dotted line) ± SD (shaded) of SC ISG transcription suggestive of minimum threshold for biological relevance. e Correlation of standard metabolic rate to log-IHNV transcriptional load; Spearman r and associated p-value provided; mean (dotted line) ± SD (shaded) of SC fish suggestive of minimum threshold for biological relevance. f Mean ± SE (bar) and individual values (dots) for standard metabolic rate (SMR) and critical oxygen threshold (O2crit) of IHNV-resistant (transcript loads < 103/μg) and IHNV-susceptible (transcript loads > 103/μg) fish relative to SC; *p < 0.05, **p < 0.01 by 1-way ANOVA and Dunnett’s multiple comparison tests