Fig. 7

Relationship between tumor cell identity and developmental GN cell origins. a Workflow for the collection of MB developed after 50 weeks in Patched^+/− mice, single-cell sequencing, and clustering analysis. n = 3 Patched^+/− mice. They are MB-1, MB-2, and MB-3. t-SNE visualization of nine clusters in 18,372 cells from three MB tumors. Cells are colored according to clusters with annotation of cell types. b Heatmap showing inferred large-scale CNVs of normal cells (GNPs/GNs at P7 WT mouse) and tumor cells of three samples. c Heatmap of mean similarity scores between MB and developmental neural lineage cells of cerebellum. d t-SNE visualization of four GN cell state scores in three MB tumors. e Relative expression of 150 genes representing SHH-MB meta-programs from combined tumor cells. Cells positive for the cell cycle program are indicated. f Jaccard similarities of the gene sets between meta-programs of tumor cells (y axis) and four cell states of granule neuron cells (x axis)