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Fig. 6. | BMC Biology

Fig. 6.

From: A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography

Fig. 6.

An example of the self-guided navigation of ROIs with automated LM/EM acquisition. a Golgi impregnated tissue sections. Example of a dataset tracked over 36 sections 3 example images imaged with 100x, 4kx, 12kx, and 2 example images of the 7 images tracked at 30kx are shown. b Manual segmentation of the cell body, nucleus, and mitochondria at 12kx using the EM information at 12kx only. c Overview and spatial relation of the self-guided navigation of the 3D stacks shown in a. d Example of fluorescent dataset acquired with our approach. 100x LM fluorescent images are overlaid on a 4kx image (insert) and the 10kx EM image stack rendered in 3D. The phase separation of FLOE1-GFP fusion protein is shown in U2OS cells. Pixel sizes in a: 100x (15.38 [pixel/μm]) for the LM, 4kx (43.03 [pixel/μm]), 12kx (129.08 [pixel/μm]), and 30kx (322.69 [pixel/μm] for the EM. Pixel sizes of EM in d: 4kx overview (43.03 [pixel/μm]) and 10 k main panel (107.58 [pixel/ μm]) LM same as in 100x in a. Values refer to the x,y-plane. Scale bars in a 100x = 10 μm; 4kx = 5 μm; 12kx = 2 μm; 30kx = 1 μm. b, d = 10 μm

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