Fig. 7
From: DNA methylation atlas and machinery in the developing and regenerating annelid Platynereis dumerilii
Decitabine treatment impairs posterior regeneration. a Graphic representation of the stages reached by control worms (normal sea water and DMSO 0.01%) and worms treated with three different concentrations of Decitabine every day for 5 days. Regeneration is delayed for 50 μM and 100 μM Decitabine conditions, from 3 days post-amputation (dpa) onwards, and for 10 μM Decitabine conditions, from 4dpa onwards. Three experiments, mean ± SD. 2-way ANOVA (p value: time p < 0.0001, treatment p < 0.0001, interaction p < 0.0001) with Tukey post hoc test (**: p < 0.01; ***: p < 0.001; ****: p < 0.0001). The number of worms used for these experiments is indicated in the figure. b Proportions of control and Decitabine-treated worms with different regeneration scores at 5dpa. Worms treated with the three different concentrations of Decitabine have been pooled. Most worms treated with Decitabine did not reach stage 5 at 5dpa, while about 50% of control worms reached this stage (some of which already having produced a first new segment). Supporting data values can be found in Additional file 2: Table S5. c Ventral views of WMISH at 5dpa of posterior part of control worms and worms treated with 50 μM Decitabine are shown. Expression of marker genes for different structures/tissues/cells during posterior regeneration has been studied, i.e., Pdum-hox3 (growth zone), Pdum-piwiB (growth zone and segmental progenitors), Pdum-pcna (proliferating cells), Pdum-caudal/cdx (pygidium and growth zone), Pdum-engrailed (Pdum-en; segmental stripes), Pdum-twist (muscle progenitors), and Pdum-neurogenin (Pdum-ngn; neural progenitors) [37]. Red arrowheads point to an expression in the growth zone, red arrows to a mesodermal expression, brown arrows to an expression at the base of the anal cirri, violet arrows to an expression in the pygidium, light blue arrows to a segmental ectodermal expression, light blue arrowheads to an expression in neural progenitors, and orange arrows to an expression in pygidial muscles. White asterisks indicate an expression in the hindgut