Fig. 8

Decitabine treatment during posterior regeneration affects subsequent post-regenerative posterior growth. a Schematic representation of the experimental design. Worms were treated with Decitabine (10 μM, 50 μM, or 100 μM) or from amputation to 5 dpa. Control animals were treated with DMSO 0.01% or put in normal seawater. After washing out, worms were kept from 5dpa to 25dpa in normal seawater and observed at several time points until 25dpa. b Graphic representation of regeneration stages that have been reached or numbers of newly added segments by Decitabine-treated and control worms. A significant delay in post-regenerative posterior growth is observed in Decitabine-treated worms as compared to controls. Two experiments, mean ± SD, 2-way ANOVA (p value: time p < 0.0001, treatment p < 0.0001, interaction p < 0.0001) with Tukey post hoc test (**: p < 0.01; ***: p < 0.001; ****: p < 0.0001). Only p values corresponding to comparison to normal seawater are shown, as highly similar ones are obtained for comparison to DMSO controls. The number of worms used for these experiments is indicated in the figure. Supporting data values can be found in Additional file 2: Table S6. c Representative morphologies at 25dpa of worms belonging to the three defined classes (see main text for details). While class 3 worms showed well-differentiated segments with parapodia (black asterisks), no or reduced parapodia were observed in class 1 and class 2 (black arrowheads) worms, respectively. Small or abnormally shaped anal cirri (blue arrows) were also frequently observed in class 1 and 2 worms