Fig. 6
From: Single-cell alternative polyadenylation analysis delineates GABAergic neuron types
Modality of poly(A) site usage in GABAergic neurons. a The distributions of poly(A) site usage for five different modalities, including distal, proximal, middle, bimodal, and multimodal. The leftmost column shows the reference distributions for modality assignment. Examples of genes with different modalities in CCKC, CHC, ISC, LPC, MNC, and PVBC are shown. B, C The table (b) depicts the numbers of genes with different modalities for each GABAergic neuron type. Accordingly, the stacked bar chart in c depicts the proportion of genes with different modalities. d The violin plots show the distributions of distal poly(A) site usage of Pak3 in different GABAergic neuron types. e The violin plots show the distribution of Pak3 gene expression level in different GABAergic neuron types. f Bimodality of distal poly(A) site usage of Pak3 could reveal different subpopulations of CHCs. The heatmap depicts the genes top correlated with distal poly(A) site usage of Pak3 cluster CHCs into two groups (Group1 vs Group2). The columns represent CHC single cells, and the rows represent correlated genes. The gradient blue to red represents gene expression level in log2(uTPM + 1). The gradient green represents distal poly(A) site usage of Pak3. g The violin plot shows the distributions of distal poly(A) site usage of Pak3 in Group1 and Group2 CHCs. h The stacked bar chart depicts the proportions of Group1 and Group2 single cells of CHCs in upper (L2/3) and deeper (L5/6) cortical layers. NA is for those single cells in which poly(A) site usage of Pak3 are not detected