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Fig. 3 | BMC Biology

Fig. 3

From: Unexpected organellar locations of ESCRT machinery in Giardia intestinalis and complex evolutionary dynamics spanning the transition to parasitism in the lineage Fornicata

Fig. 3

Characterization of GiVPS25-HA subcellular location. A Trophozoite cell periphery and cytosol. (I) Ventral and (II) middle optical slice of transgenic Giardia trophozoite expressing epitope-tagged GiVPS25-HA (green). All images were obtained using Laser Scanning Confocal Microscopy. All scale bars: 5 μm. B PVs. (I) Immunofluorescence assay of transgenic Giardia trophozoite labelled for epitope-tagged GiVPS25-HA (green) and Dextran-TexasRed (magenta). (II) Distribution of co-localization parameters for GiVPS25-HA and Dextran-TexasRed labeling from ≥ 15 analysed cells. Mean values for each parameter are indicated. (III) Signal overlap analysis and co-localization coefficients calculated for all slices of the sample either for the whole cell or ROI. Scale bars: composite 5 μm and ROI 1 μm. All images were obtained using Laser Scanning Confocal Microscopy

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BMC Biology

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