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Fig. 5 | BMC Biology

Fig. 5

From: Unexpected organellar locations of ESCRT machinery in Giardia intestinalis and complex evolutionary dynamics spanning the transition to parasitism in the lineage Fornicata

Fig. 5

Co-labelling of GiVPS25-HA, GiVPS36A-HA, and GiHA-VPS20L with ER membrane marker GiPDI2. A HA-GiVPS20L is found in the cytosol and punctate structures. Scale bars: 5 μm. B–D Panels I: Co-labelling of PDI2 (magenta) in cells expressing either B GiVPS25-HA (green), C GiVPS36A-HA (green), or D HA-GiVPS20L (green). B–D Panels II: Mean values from ≥ 15 analysed cells for each parameter are indicated. B–D Panels III: Signal overlap analysis and co-localization coefficients calculated for all slices of the sample either for the whole cell or ROI. Scale bars: composite 5 μm and ROI 1 μm. All images were obtained using Laser Scanning Confocal Microscopy

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BMC Biology

ISSN: 1741-7007

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