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Fig. 6 | BMC Biology

Fig. 6

From: Unexpected organellar locations of ESCRT machinery in Giardia intestinalis and complex evolutionary dynamics spanning the transition to parasitism in the lineage Fornicata

Fig. 6

Co-expression of epitope-tagged GiVPS25 with either GiVPS20L or GiVPS36. Microscopy analysis of cells co-expressing GiVPS25HA (green) with either A GiVPS36A-V5 (magenta) or B GiV5-VPS20L (magenta). Panels I: representative cell images and percentage of co-labeling. Panels II: Signal overlap analysis in both whole cells and regions of interest (ROI). Scale bars: (I) 10 μm, (II whole cell) 5 μm, and (II-ROI) 1 μm. Panels III: Mean values from ≥ 15 analysed cells for each parameter are indicated. All images were obtained using Laser Scanning Confocal Microscopy

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