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Fig. 8 | BMC Biology

Fig. 8

From: Unexpected organellar locations of ESCRT machinery in Giardia intestinalis and complex evolutionary dynamics spanning the transition to parasitism in the lineage Fornicata

Fig. 8

Characterization of GiCHMP7 subcellular location. A Immunofluorescence assays of HA-GiCHMP7-expressing cells yield a diffused punctate pattern with elements of perinuclear ER staining (arrowhead). Scale bars: 5 μm. B (I) Co-labelling of HA-GiCHMP7 (magenta) -expressing cells with GiPDI2 (green). (II) Distribution of co-localization parameters for HA-GiCHMP7 and GiPDI2 labeling from ≥ 15 analysed cells. Mean values for each parameter are indicated. (III) Signal overlap analysis for all slices of the sample either for the whole cell or ROI. C HA-GiCHMP7 is associated to Giardia mitosomes. (I) Co-labelling of HA-GiCHMP7 (magenta) -expressing cells with GiIscU (green). (II) Distribution of co-localization parameters for HA-GiCHMP7 and GiIscU labeling from ≥ 15 analysed cells. Mean values for each parameter are indicated. (III) Signal overlap analysis for all slices of the sample either for the whole cell or ROI. Scale bar: composite 5 μm and ROI 1 μm. All images were obtained using Laser Scanning Confocal Microscopy

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