Fig. 7

Functional differences in neurons derived from these models were assessed by voltage clamp analysis. a Quantitation of neuron soma area revealed a larger soma size in UM-derived neurons. Left: representative confocal images comparing the AP and UM models, with arrows highlighting a cell soma; Right: quantitation of neuron soma area for the three models. Data is derived from three biological replicate experiments (n = 3) using two clonal lines for the AP and UM models, and one clonal line for the UC-M model. Data values are in Additional file 4. Plot shows the median value, calculated by using a Kruskal-Wallis non-parametric test as described in the “Methods” section. b UM-derived neurons exhibit significantly higher cell capacitance and lower input resistance than UC-M- or AP-derived neurons. c Steady-state outward current density at 0mV was significantly greater for UC-M- than for UM- or AP-derived neurons. Whole-cell inward and outward current density for currents were evoked by a voltage step from −80 to 0 mV. d Collectively, iPSC-derived cINs from all three subject-derived models exhibited higher input resistance than cExNs. e Whole-cell currents were evoked by 500 μM Choline or ACh in neurons, with representative data shown for the UC-M (left) versus AP (right) models. f The integrated ACh-evoked current density was significantly smaller for AP-derived neurons and g the ACh/choline ratio for integrated current was smaller for both AP- and UM-derived neurons, by comparison with UM-C. Data were derived from three biological replicate experiments (n = 3), which used two clonal lines for the AP and UM models, and one clonal line for the UC-M model. Box plots show combined data from cExNs and cINs except in panel (d). P <0.05, was determined by 2-way ANOVA on ranks with post hoc Student-Newman-Keuls test. A complete summary of the physiological recordings performed is provided in Additional file 13