Table 1 O₂ consumption rates (states 3 and 4) for isolated mitochondria from quadriceps

From: Age-related susceptibility to insulin resistance arises from a combination of CPT1B decline and lipid overload

Tissue		Substrates
Tissue		Pyruvate		Pyruvate/glutamate		Palmitoyl-CoA/carnitine
Quadriceps		Young	Old	Young	Old	Young	Old
State 3	LFD	428 ± 56	424 ± 58	476 ± 68	458 ± 57	131 ± 29	153 ± 20
State 3	HFD	524 ± 56	415 ± 48	627 ± 77	419 ± 43	204 ± 14*	184 ± 20*
State 4	LFD	20.9 ± 5.9	20.2 ± 4.5	21.9 ± 6.8	30.2 ± 9.7	36.1 ± 10.2	26.5 ± 3.6
State 4	HFD	20.2 ± 2.6	18.9 ± 3.7	27.6 ± 8.1	14.9 ± 2.2	35.6 ± 4.4	26.1 ± 5.1
RCR	LFD	26.0 ± 4.7	26.3 ± 3.7	38.6 ± 13.1	25.3 ± 3.9	4.2 ± 0.8	6.1 ± 0.5
RCR	HFD	28.2 ± 5.1	27.0 ± 4.0	30.3 ± 10.9	31.8 ± 3.9	5.9 ± 0.5	7.6 ± 0.8

All substrates were added in the presence of malate. Values are shown as mean ± SEM (nmol ⋅ mg mitochondrial protein⁻¹ ⋅ min⁻¹). State 3: ADP-stimulated maximal respiration. State 4: carboxyatractyloside-induced inhibition of ATP synthesis (via ATP-ADP translocase inhibition), RCR: respiratory control ratio (State 3/State 4), *p_diet = 0.025

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ISSN: 1741-7007

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