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Fig. 1 | BMC Biology

Fig. 1

From: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

Fig. 1

Characterization of Rib FP enzymes using SPT. A EAMSD versus time plot for the Rib FP enzymes RibDG-, RibE-, RibAB-, and RibH-mV. All SPT data taken for the EAMSD analysis and obtained results are summarized in Tables 1 and 2. B, C Bright field images of cells expressing ribAB-mV or ribH-mV from the original gene loci in exponential growth phases overlaid with trajectories derived from the according SPT datasets. Examples of trajectories (well separated examples were chosen) are shown in red. Scale bar represents 1 μm and is valid for both image montages. D Spot location heat maps displaying the spatial distributions of trajectories for all four Rib FP enzymes. Trajectories were projected onto the two dimensional cytoplasmic area of a normalized cell which resembles an averaged sized cell of B. subtilis in mid-exponential growth phase (1 μm × 3 μm). The likelihood of finding trajectories at a certain place in the cytoplasm is indicated by a color code from blue to red (indicated below the cell maps). Signal intensities of spatial distribution maps have been normalized with each other. E Speed maps displaying the spatial distributions of the average single step diffusions for all four Rib-mV fusion enzymes binned over areas of 0.1 μm2 for normalized cells

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