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Fig. 3 | BMC Biology

Fig. 3

From: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

Fig. 3

Comparison of diffusion and localization of RibAB-mV with and without RibH being present in the cytoplasm. For calculation of overall DCs from EAMSD, as well as, for JD analysis, we took a comparable number of trajectories (Tables 1 and 2). A Analysis of JD distribution for RibAB-mV in the presence and absence of RibH. JD distributions were fitted using two (left panel), one (middle panel), or two (right panel) Rayleigh distributions. The fitting algorithm used was the same as for RibDG-mV. B Model prediction versus observation plots for Rayleigh distribution modeling of JDs in A are displayed. C Bubble plots displaying relative proportions of diffusive populations and their according DCs (Table 3). D EAMSD analysis after five lag times for RibAB-mV in the presence (~ 0.63 μm2/s) and absence (~ 0.64 μm2/s) of RibH. E Speed map representations of RibAB-mV either in the presence or absence of RibH displaying the spatial distributions of single step diffusion binned over areas of 0.1 μm2 for normalized cells. F Subcellular analysis of confined and free trajectories for RibAB-mV either in the presence or absence of RibH. Displayed are the results using the radii given in Table S2 with three, six, or nine consecutive steps of confinement. The normalized probability is given below the normalized cell representations

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