Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

Fig. 4

RibH-mV reveals three different subpopulations that change upon deletion of the gene encoding for interacting RibE. A EAMSD analysis of two strains expressing fluorescent RibH-mV in the presence or absence of RibE. In case of the ribE deletion strain results including outliers and results after their removal are shown. B Comparative JD analysis for the same strains as in A shows the existence of three diffusive subpopulations for RibH-mV. Those different subpopulations have been colored according to their mean DC derived from JD analysis (Table S2). Subpopulations in JD distributions were fitted using up to three Rayleigh distributions either fitted independently or simultaneously. C Model prediction versus observation plots for Rayleigh distribution modeling of JDs in B. D Bubble plot illustrating differences in relative proportions of subpopulations with respect to DCs derived from JD analysis. E Proposed model for encapsulation of RibE by RibH pentamers assuming either an equilibrium between free and encapsulated RibE (posttranslational mechanism), a cotranslational encapsulation mechanism, or a mixture of both mechanisms. F Speed map representations of RibH-mV either in the presence or absence of RibE displaying the spatial distributions of single step diffusion binned over areas of 0.1 μm2 for normalized cells. G Subcellular analysis of confined and free trajectories for RibH-mV either in the presence or absence of RibE and further with outliers removed for the deletion mutant. Displayed are the results using the radii given in Table S2 with three, six, or nine consecutive steps of confinement. The normalized probability is given on the right to the normalized cell representations

Back to article page