Fig. 7

Mode of diffusion analysis for RibH-mV SPT data and comparison with synthetic data. A Individual TAMSD plots of trajectories derived from the RibH-mV SPT dataset sorted according to their mode of diffusion with inserted spot location heat maps displaying the localization of spots for those trajectories in a normalized cell representation. Signal intensities of spot location heat maps have been normalized with each other, and an absolute number of classified trajectories are given in brackets within the plot. B Image montage of a bright field microscopy picture overlaid with trajectories from exponentially grown PY79 expressing ribH-mV. Brownian trajectories are colored in blue; the four different identified superdiffusive trajectories in this particular cell are displayed in yellow, pink, red, and green. Scale bar represents 1 μm. C TAMSD plot for all trajectories displayed in the left cell of panel B. TAMSD curves of trajectories classified as Brownian are colored in blue, superdiffusive trajectories are colored as before in B. Shown are trajectories with R² values that are at least 0.9 or higher (indicated in G as a red line). D Log-log plot showing TAMSD curves for superdiffusive trajectories shown in B. Colors are the same as before. E Probability plots showing the normalized probability of finding exponent α in our RibH-mV SPT data set (upper one with arrows) as well as for the synthetic data (lower one) generated with the parameters taken from JD analysis of the same dataset. Colors used are the same as in A. F Probability distribution of exponent alpha independently from its generalized DC for RibH-mV SPT data (upper one) as well as for the synthetic data. G Probability histograms displaying the likelihood of having an R² value for individual fits of all trajectories from the RibH-mV dataset used before in F as well as for the synthetic data. The red line marks the threshold that has been used for selection of trajectories shown in C. H Subcellular analysis of confined and free trajectories for RibH-mV. Displayed are the results using the radius given in Table S2 with six or nine consecutive steps of confinement. The normalized probability is given below the normalized cell representations. I, J EAMSD plots with linear and exponential fitting for trajectories with at least six (I) or nine (J) consecutive steps classified as confined shown in H. The legend shown in I is also valid for J with indicated standard error of the mean (SEM)