Table 3 Summary of diffusion coefficients (DCs) derived from EAMSD analysis using five lag times for all four Rib FP enzymes
Gene | Enzyme function(s) | MM of native monomer (MM of FP fusion [kDa]) | Oligomeric state and MM of oligomer (MM of FP oligomer [kDa]) | Diffusion [μm2/s] from EAMSD (R-value) | N of cells (N of movies) | N of trajectories per cell (N of total trajectories) | Average lifetime [s] (frames) |
|---|---|---|---|---|---|---|---|
ribDG (-mV) | DAROPP deaminase/AROPP reductase | 39.15 (66.82) | Tetramer 156.6 (267.29) | 0.657 (0.994) | 144 (49) | 44 (5860) | 0.092 (6.7) |
ribE (-mV) | RF synthase | 23.33 (50.99) | Trimer 69.99 (152.99) | 0.570 (0.994) | 94 (36) | 70 (5943) | 0.11 (7.7) |
ribAB (-mV) | GTP cyclohydrolase II/DHBP synthase | 43.96 (71.64) | Dimer 87.92 (143.28) | 0.646 (0.992) | 179 (55) | 49 (8161) | 0.1 (7.3) |
ribH (-mV) | DMRL synthase | 16.14 (43.80) | 1. 60-mer 968.4 (2628.23) 2. Pentamer 80.7 (219.02) | 0.352 (0.997) | 76 (25) | 143 (10,679) | 0.14 (10) |