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Table 3 Summary of diffusion coefficients (DCs) derived from EAMSD analysis using five lag times for all four Rib FP enzymes

From: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

Gene Enzyme function(s) MM of native monomer (MM of FP fusion [kDa]) Oligomeric state and MM of oligomer (MM of FP oligomer [kDa]) Diffusion [μm2/s] from EAMSD (R-value) N of cells (N of movies) N of trajectories per cell (N of total trajectories) Average lifetime [s] (frames)
ribDG (-mV) DAROPP deaminase/AROPP reductase 39.15 (66.82) Tetramer 156.6 (267.29) 0.657 (0.994) 144 (49) 44 (5860) 0.092 (6.7)
ribE (-mV) RF synthase 23.33 (50.99) Trimer 69.99 (152.99) 0.570 (0.994) 94 (36) 70 (5943) 0.11 (7.7)
ribAB (-mV) GTP cyclohydrolase II/DHBP synthase 43.96 (71.64) Dimer 87.92 (143.28) 0.646 (0.992) 179 (55) 49 (8161) 0.1 (7.3)
ribH (-mV) DMRL synthase 16.14 (43.80) 1. 60-mer 968.4 (2628.23)
2. Pentamer 80.7 (219.02)
0.352 (0.997) 76 (25) 143 (10,679) 0.14 (10)