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Table 4 Summary of DCs determined by EAMSD or JD analysis (independent fitting)

From: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

Strain relevant genotype Diffusion [μm2/s] from EAMSD (R-value) D1 [μm2/s] (Pop. %) D2 [μm2/s] (Pop. %) D3 [μm2/s] (Pop. %) N of cells (N of movies) N of trajectories per cell (N of total trajectories) Av. life time [s] (frames) Av. cell length [μm]
ribDG-mV 0.657 (0.994) 0.234 ± 0.002 (7.9%) 1.16 ± 0.001 (92.1%) 144 (49) 44 (5860) 0.092 (6.7) 2.86
ribDG-mV (ΔribH) 0.682 (0.994) 0.162 ± 0.001 (7.69%) 1.14 ± 0.001 (92.3%)   126 (44) 52 (5918) 0.1 (7.4) 2.87
ribAB-mV 0.646 (0.992) 0.0966 ± 0.001 (2.6%) 1.17 ± 0 (97.4%) 179 (55) 49 (8161) 0.1 (7.3) 2.74
ribAB-mV (ΔribH) 0.553 (0.994) 1.02 ± 0 (100%) 65 (19) 115 (7262) 0.11 (8) 2.82
ribE-mV 0.570 (0.994) 0.0287 ± 0.001 (1.3%) 0.514 ± 0.008 (14%) 1.12 ± 0.002 (84.7%) 94 (36) 70 (5943) 0.11 (7.7) 2.69
ribE-mV (ΔribH) 0.691 (0.994) 1.3 ± 0 (100 %) 316 (121) 19 (5868) 0.091 (6.6) 2.75
ribH-mV 0.352 (0.997) 0.0815 ± 0 (10%) 0.375 ± 0 (65%) 1.01 ± 0.001 (25%) 76 (25) 143 (10,679) 0.14 (10) 2.80
ribH-mV (ΔribE, incl. outliers) 0.203 (0.989) 0.0164 ± 0 (16%) 0.168 ± 0 (37%) 0.684 ± 0.001 (47%) 80 (27) 133 (10,637) 0.17 (13) 2.81
ribH-mV (ΔribE, excl. outliers) 0.237 (0.993) 0.0319 ± 0 (9.1%) 0.18 ± 0 (40%) 0.691 ± 0.001 (50.9%) 79 (27) 134 (10,622) 0.16 (11) 2.82
ribH-mV (control) 0.329 (0.997) 0.0855 ± 0 (11.3%) 0.37 ± 0 (66.1%) 1.04 ± 0.001 (22.6%) 239 (83) 148 (33,386) 0.14 (10) 2.86
ribH-mV (30 min Rifampicin) 0.463 (0.997) 0.0665 ± 0 (3.79%) 0.475 ± 0 (64.2%) 1.11 ± 0.001 (32.01%) 227 (78) 161 (34,352) 0.13 (9.7) 2.73
ribAB-mV (ectopic) 0.653 (0.994) 1.13 ± 0.001 (94.4%) 0.343 ± 0.004 (5.65%) 201 (60) 51 (10,342) 0.12 (7.4) 2.70
ribAB-mV (ectopic, ΔribH) 0.532 (0.995) 0.913 ± 0 (100%) 65 (27) 172 (11,186) 0.1 (8.5) 2.70
ribE-mV (ectopic) 0.589 (0.995) 1.1 ± 0.002 (98.8% ± 0.004) 0.539 ± 0.067 (98.8% ± 0.004) - 95 (30) 126 (10,690) 0.11 (7.8) 2.90
ribE-mV (ectopic, ΔribH) 0.556 (0.995) 1.08 ± 0 (100%)    63 (31) 178 (10,265) 0.11 (7.8) 2.88
ribE-mV (ectopic, ΔribE) 0.514 (0.994) 0.917 ± 0 (100%)    111 (45) 102 (10,612) 0.11 (8.2) 2.69