Fig. 3

The OptoArm provides the light intensity and stability required for optogenetic experiments. A The different lenses used for testing. B A schematic outlining the tested parameters with the three different lenses. C Raster showing the x,y coordinates used to measure the intensity of the LED. D The different integrated intensity profiles of the LED with and without lenses. The histograms show the zoomed-in distribution of intensity readings between a minimum and maximum intensity with a specified bin width. Red lines show the average intensity. E Smoothened histograms of D that show the increase in intensity when using different lenses with the OptoArm. F Schematic showing the method to assess the stability of intensity over time. G Distributions of intensity readings at the different timepoint without a lens (H) and with a 10° lens. All readings were performed at 448 nm