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Fig. 7 | BMC Biology

Fig. 7

From: An economical and highly adaptable optogenetics system for individual and population-level manipulation of Caenorhabditis elegans

Fig. 7

Mutations in the cholinergic or GABAergic system affect ∆body length after optogenetic stimulation with the OptoArm. A A schematic showing the different mutant used to verify previously established results. B Schematic of the experimental outline: the change in body length was measured after blue light stimulation with an intensity of 1.6 mW/mm2. C Kinetics of optogenetic stimulation in mean ± SEM body length of control worms and unc-26(s1710) and unc-47(e307) mutants expressing ChR2 in cholinergic neurons, n = 9–10. A perimeter approach was used. D Quantification of mean ± SEM normalized body length of control D1 worms and unc-26(s1710) and unc-47(e307) mutants expressing ChR2 in cholinergic neurons. Two-way ANOVA (Interaction, ATR, Genotype: p < 0.001) with post hoc Dunnett, n = 11–15 for ATR+ and n = 5–6 for ATR−. A midline approach was used. E Kinetics of optogenetic stimulation in mean ± SEM normalized body length of control worms and unc-26(s1710) and unc-47(e307) mutants expressing ChR2 in GABAergic neurons, n = 9-11. A perimeter approach was used. F Quantification of mean ± SEM normalized body length of control worms and unc-26(s1710) and unc-47(e307) mutants expressing ChR2 in GABAergic neurons. Two-way ANOVA (Interaction, ATR, Genotype: p < 0.001) with post hoc Dunnett, n = 10–15 for ATR+ and n = 5–6 for ATR−. A midline approach was used. G Long-term photo-stimulation of control worms, unc-26(s1710) and unc-47(e307) mutants expressing ChR2 in cholinergic neurons. Two-way repeated ANOVA with Geisser-Greenhouse correction (time, genotype, time × genotype and individual worms: p < 0.001) and post hoc Dunnett, n = 10. A midline approach was used. Blue bars represent “light ON.” Acetylcholine: zxIs6 (Punc-17::ChR2::YFP), zxIs3 (Punc-47::ChR2::YFP). *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001. Error bars represent S.E.M

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