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Table 1 Specifications overview for stains and antibodies used in the assay and microscope filters and spectra settings

From: A phenomics approach for antiviral drug discovery

Label

Excitation spectra (nm)

Emission spectra (nm)

Source

Cat-No

Stock concentration

Concentration used

Target

Hoechst 33342

377/50

447/60

Invitrogen

H3570

10 mg/mL

10 μg/mL

DNA (nucleus)

Pan coronavirus Monoclonal Antibody (FIPV3-70)

-

-

Invitrogen

11500123

1 mg/mL

1:1000

Coronavirus viral nucleoprotein

Goat Anti-Mouse IgG H&L secondary antibody

628/40

692/40

Invitrogen

10739374

2 mg/mL

1:500

Wheat Germ Agglutinin Alexa Fluor™ 555 Conjugate

562/40

624/40

Invitrogen

W32464

5 mg/mL

15 μg/mL

Golgi, plasma membrane (sialic acid and N-acetylglucosamine moieties of glycoproteins)

Phalloidin Alexa Fluor™ 568 conjugate

Invitrogen

A12380

40x (200U)

10 μL/mL

F-actin

SYTO 14 green

531/40

593/40

Invitrogen

S7576

5 mM

4 μM

Nucleoli, cytoplasmic RNA

Concanavalin A/Alexa Fluor 488 conjugate

482/35

536/35

Invitrogen

C11252

5 mg/mL

80 μg/mL

Endoplasmic reticulum (mannose residues of glycoproteins)