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Table 1 Specifications overview for stains and antibodies used in the assay and microscope filters and spectra settings

From: A phenomics approach for antiviral drug discovery

Label Excitation spectra (nm) Emission spectra (nm) Source Cat-No Stock concentration Concentration used Target
Hoechst 33342 377/50 447/60 Invitrogen H3570 10 mg/mL 10 μg/mL DNA (nucleus)
Pan coronavirus Monoclonal Antibody (FIPV3-70) - - Invitrogen 11500123 1 mg/mL 1:1000 Coronavirus viral nucleoprotein
Goat Anti-Mouse IgG H&L secondary antibody 628/40 692/40 Invitrogen 10739374 2 mg/mL 1:500
Wheat Germ Agglutinin Alexa Fluor™ 555 Conjugate 562/40 624/40 Invitrogen W32464 5 mg/mL 15 μg/mL Golgi, plasma membrane (sialic acid and N-acetylglucosamine moieties of glycoproteins)
Phalloidin Alexa Fluor™ 568 conjugate Invitrogen A12380 40x (200U) 10 μL/mL F-actin
SYTO 14 green 531/40 593/40 Invitrogen S7576 5 mM 4 μM Nucleoli, cytoplasmic RNA
Concanavalin A/Alexa Fluor 488 conjugate 482/35 536/35 Invitrogen C11252 5 mg/mL 80 μg/mL Endoplasmic reticulum (mannose residues of glycoproteins)