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Table 1 List of taxa used in this study and summary of sequencing and assembly. Under Strain or Pubmed ID, “CC” refers to Chlamydomonas Culture Collection at the University of Minnesota (CC, USA), “F” refers to Culture Collection of Freshwater Algae at the Institute of Hydrobiology, Chinese Academy of Sciences (FACHB, China), “N” refers to National Institute for Environmental Studies (NIES, Japan), “S” refers to Culture Collection of Algae at the University of Göttingen (SAG, Germany), and “U” refers to The Culture Collection of Algae at the University of Texas at Austin (UTEX, USA). “QRPMK” and “TR” under RNA Extraction Method refer to QIAGEN RNeasy Plant Mini Kit and TRizol RNeasy, respectively. Strains assigned an asterisk represent data from previously published studies, with accession numbers shown in Supplementary Materials: Table S1 [21, 47]

From: Phylotranscriptomics points to multiple independent origins of multicellularity and cellular differentiation in the volvocine algae

Taxa Strain or Pubmed ID RNA extraction method Total # of raw reads Total # of clean PE reads Total # of Contigs N50 G/C %
Astrephomene gubernaculifera N-418 QRPMK 61460551 44350080 78105 3894 0.61
A. perforata N-564 QRPMK 63350620 46811342 72913 3613 0.61
Basichlamys sacculifera N-566 QRPMK 66205749 48327288 134880 1945 0.69
Colemanosphaera angeleri F 2363*   28119810 27000176 120763 3348 0.62
C. charkowiensis F 2326*   27967726 26876865 103286 3382 0.62
  N-3383 TR 58777161 40550696 119848 2523 0.62
Chlamydomonas debaryana S 11-55a QRPMK 87455695 64552440 105096 2776 0.67
  S 70.81 QRPMK 50406749 42095108 103962 2858 0.64
C. globosa S 81.72 TR 73820020 49419928 120062 2079 0.65
C. moewusii S 11-16f QRPMK 54728573 40436359 118833 2498 0.6
C. reinhardtii CC-503*       
C. schloesseri S 2486 QRPMK 70163393 49255021 64233 3094 0.66
Chlorella variabilis NC64a 20852019*       
Eudorina cylindrica F 2322*   26442110 25262148 103026 2846 0.64
  N-722 QRPMK 44479173 36297801 105029 2927 0.64
E. elegans F 2321*       
  N-456 TR 84549961 56756776 122975 2108 0.63
  N-458 QRPMK 67451343 46725012 131335 2944 0.65
  N-568 QRPMK 83433797 69539684 120487 3614 0.64
  N-717 QRPMK 47625429 37616922 89118 2094 0.64
  N-719 QRPMK 57157324 43565312 98180 2976 0.63
  N-720 QRPMK 38134498 31388413 95662 3699 0.61
E. illinoisensis N-460 QRPMK 62485922 49396583 97014 2615 0.64
E. minodii N-856 QRPMK 83574258 66413244 128802 4127 0.61
E. peripheralis N-725 QRPMK 46607143 36528930 100934 2322 0.62
E. unicocca S 24-1c TR 54482446 37679863 110391 2267 0.62
Gonium multicoccum N-737 QRPMK 65984190 44301578 122279 3275 0.64
G. pectorale N-2863*       
G. octonarium N-851 TR 60029472 39826211 104857 2302 0.65
G. quadratum N-653 QRPMK 62431743 48230702 100468 3789 0.64
G. viridistellatum N-654 QRPMK 43286980 31852410 97991 2837 0.65
Pandorina colemaniae F 2361*   25181003 23823370 80843 3292 0.62
  N-572 TR 49862534 37589048 84053 2237 0.62
P. morum F 2362*   29837807 28442854 147990 2674 0.61
  N-890 QRPMK 39337532 24490235 133742 3123 0.61
Platydorina caudata N-728 QRPMK 48702213 36896556 125206 3503 0.61
Pleodorina indica N-736 QRPMK 57564627 44082516 192570 2006 0.63
P. japonica U 2523 QRPMK 64897803 49495315 83662 3499 0.61
P. starrii N-1362 TR 68790016 45935282 87641 2438 0.64
  N-1363 TR 59466876 44726861 128732 1695 0.63
P. thompsonii N-4126 QRPMK 81077847 61986735 118940 4005 0.62
Tetrabaena socialis N-571*   42302450 41232209 62014 1012 0.65
Vitreochlamys aulata N-878 QRPMK 80710519 54651118 132115 2673 0.65
  S 80.81 QRPMK 41008694 32585611 84834 2100 0.65
V. nekrassovii S 11-10 QRPMK 65658295 48341108 109360 3304 0.62
V. ordinata N-882 TR 60631467 42981449 75434 2778 0.69
Volvox africanus N-863 QRPMK 70739371 52378949 110066 4586 0.55
V. aureus N-541 QRPMK 80821130 64315625 106245 4065 0.55
V. barberi N-730 QRPMK 49805303 37197105 152910 2217 0.58
V. carteri f. kawasakiensis N-732 TR 53994112 34239609 86004 1982 0.58
V. carteri f. nagariensis 20616280*       
  N-865 QRPMK 65624222 50641434 117314 3947 0.57
V. carteri f. weismannia N-866 QRPMK 25913610 20987137 78230 2636 0.57
V. dissipatrix N-4128 QRPMK 77137081 51045569 112190 5146 0.55
V. ferrisii N-3986 QRPMK 49810357 37522413 91045 3281 0.57
V. gigas N-867 QRPMK 50554912 37592146 151302 2499 0.63
V. globator S 199.80 QRPMK 67413919 52861449 100861 4005 0.56
V. kirkiorum N-543 QRPMK 44941633 34650729 89533 3081 0.56
V. obversus N-868 QRPMK 67394604 50823546 99171 3881 0.58
V. ovalis N-2569 QRPMK 52242003 35319700 107999 3643 0.53
V. powersii N-4127 QRPMK 55232784 42410688 140237 2908 0.62
V. tertius N-544 QRPMK 86222840 62563240 108551 5454 0.54
Volvulina boldii N-893 QRPMK 25665262 20161297 158265 1593 0.62
V. compacta F 2337*   27543035 26374210 90261 3550 0.61
  N-582 QRPMK 53045384 38991781 112583 3380 0.63
V. pringsheimii N-895 TR 82233959 55849890 128304 3122 0.62
V. steinii S 90-1 QRPMK 46133638 37699635 89515 3635 0.62
Yamagishiella unicocca F 2364*   30922957 29574420 64415 3609 0.63