Fig. 3

Pharyngeal organ-specific gene expression in Pitx TALEN2^−/− mutants. In situ hybridization to Pitx mutants at N5 and T1 stage for Lhx3 (a, a’, b, b’), Prop1 (c, c’, d, d’), and Pit (e, e’, f, f’). g, g’ In situ hybridization to Pitx mutants at T1 stage for Pou4. Arrowheads indicate the preoral pit region, arrows indicate the mouth region. In situ hybridization to Pitx mutants at N5 and T1 stage for Nkx2.1 (h, h’, i, i’), Hex (j, j’, k, k’), FoxE4 (l, l’, m, m’), and Krox (n, n’, o, o’). In i’, the black arrowhead shows the expression boundary of Nkx2.1 on the right side and red purple arrowhead shows expressing boundary on the left side. All images are dorsal views. Numbers in the bottom right corner of a panel show the number of times the phenotype depicted was observed, out of the total number of embryos from that genotype analyzed. A, anterior; P, posterior; L, left side; R; right side. Scale bars: 50 μm