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Fig. 5 | BMC Biology

Fig. 5

From: Pitx controls amphioxus asymmetric morphogenesis by promoting left-side development and repressing right-side formation

Fig. 5

Partial loss of Pitx function exhibits a mild phenotype and disturbs pharyngeal organs asymmetry. a, b Left lateral view of the L3 larvae (3-gill slits). a’, b’ The larvae are shown in a view between right lateral and ventral view, which clearly shows that the endostyle and CSG are symmetrical in Pitxa/c homozygotes (b’). m, mouth; pp: preoral pit; en, endostyle; csg, club-shaped gland; g: gill slit. In situ hybridization to WT/heterozygotes and Pitxa/c homozygotes at N5 and T1 stage for Lhx3 (c, c’, d, d’), Prop1 (e, e’, f, f’), and Pit (g, g’, h, h’). i, i’ In situ hybridization to WT/heterozygotes and Pitxa/c homozygotes at T1 stage for Pou4. Black arrowheads indicate the anterior preoral pit region and red arrowheads indicate the posterior preoral pit region , arrows indicate the mouth region. In situ hybridization to WT/heterozygotes and Pitxa/c homozygotes at N5 and T1 stage for Nkx2.1 (j, j’, k, k’), Hex (l, l’, m, m’), FoxE4 (n, n’, o, o’), and Krox (p, p’, q, q’). In (k’), the black arrowhead shows the expression boundary of Nkx2.1 on the right side and red purple arrowhead shows expressing boundary on the left side. Images are dorsal views in c–q’. Numbers in the bottom right corner of a panel show the number of times the phenotype depicted was observed, out of the total number of embryos from that genotype analyzed. A, anterior; P, posterior; D, dorsal; V, ventral; L, left side; R; right side. Scale bars: 50 μm

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