Fig. 6

Arrangement of mesoderm-derived organs and peripheral nerves in partial loss of Pitx function. In situ hybridization to WT/heterozygotes and Pitxa/c homozygotes at N5 stage for Hand (a, a’), Gata1/2/3 (b, b’), Pdvegfr (d, d’), and Scl (e, e’). Blue arrowheads are Hatschek’s nephridium and black arrowheads are hematopoietic region. Black arrows are the HRD. c, c’ The arrangement of somites is marked by m-actin in N5 stage embryos. Longitudinal dashed lines indicate notochord, transversal dashed lines mark somite outlines. f, f’ Yellow arrows indicate peripheral nerves marked by acetylated α-tubulin. Quantification of the distance between a pair of somites (g), and the distance between a pair of peripheral nerves (h). Posterior five pairs of somites are used for measure in one embryo, and four pairs of peripheral nerves shown in the image are used for measure. Significance determined by t-test is indicated by asterisks: **P < 0.01. Embryos are all dorsal views. Numbers in the bottom right corner of a panel show the number of times the phenotype depicted was seen, out of the total number of embryos from that genotype analyzed. A, anterior; P, posterior; L, left side; R; right side. Scale bars: 50 μm