Fig. 3

Primary cell wall cellulose-deficient mutants exhibit enhanced resistance to Fo5176. A, B Root vascular penetration of WT (Col-0 in (A) and Col-0 JGP in (B)) and primary cellulose deficient mutants at various days post-treatment (dpt) with Fo5176 pSIX1::GFP microconidia. Values represent the mean ± standard error of at least 3 independent experiments, each one containing at least 12 seedlings. Statistical significance calculated via repeated measures two-way ANOVA with Tukey post-hoc test (p value ≤ 0.05 (genotype), p value ≤ 0.05 (time), p value ≤ 0.05 (genotype x time)). Significant differences at the last time point shown (7 dpt) are indicated on the graph using letters; statistics of remaining time points summarized in Additional file 9: Table S4 A-F. C, D Quantification of Fo5176 pSIX1::GFP colonies after surface sterilization of infected roots at 7dpt. Box plots: centerlines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend to the minimum and maximum. N ≥ 3 independent experiments, each one containing at least 6 roots. Statistical significance was calculated via one-way ANOVA with a Tukey post-hoc test (p value ≤ 0.05)