Fig. 2.

“Incompatible” fused cells (via CATs) often escape incompatibility-triggered cell death. a Examples of the four observed types of cellular behavior following CAT-mediated fusion of “compatible” and “incompatible” cells. Strains shown: Ls.17 H1-mCherry paired with Cf.38 H1-sGFP (I), Ls.17 H1-sGFP (II), PH H1-sGFP (III), and BB H1-sGFP (IV). Arrows: nuclei undergoing degradation; arrowheads: migrating nuclei; asterisks: cell shrinkage. Bars = 5 μm. b Frequencies of the four types of cellular behavior in self, “compatible”, and “incompatible” pairings. The numbers of detected events in each case are provided in brackets (detailed results in Additional file 2: Table S2). c Frequency of nuclear migration through CATs in viable heterokaryons resulting from self, “compatible”, and “incompatible” pairings. Bars = SD. In b, c, statistical significance of differences between the compared groups was tested with one-way ANOVA followed by Tukey’s post hoc test; in b, groups marked by the same superscript character did not differ significantly; in c, ** p value ≤ 0.01; ns: non-significant (p value > 0.05)