Fig. 5

Endothelial cells accumulate SRSF1/3 and SRPK1 proteins upon FGF-2 treatment in an in vivo sponge assay. a Cellulose sponges loaded with FGF-2 or with PBS were engrafted under the skin of mice (n=6/group). As indicated in the graph, cellulose sponges were repeatedly injected. After 7 days, the sponges were collected and lysed for total protein extraction. b Representative immunoblots of SRPK1, SRPK2, SRSF1, and SRSF3 proteins from sponges treated with PBS or FGF-2. Tubulin was used as a loading control. c Semi-quantification using ImageJ software of FGF-2 effects on the indicated proteins relative to tubulin signals. The ratio obtained in PBS condition was arbitrarily assessed the value 1. Graphs represent mean values ± SD of 3 independent protein extracts (t test, *p<0.05, ***p<0.001, ns: not significant)