Fig. 2

Phylogenetic analysis of Dfr ORF1/2. a, b Phylogenetic trees of Dfr ORF1 (a) and ORF2 (b), constructed using the neighbor-joining method, depicting real branch distances between ORFs of denoted species. Note that non-dipteran species included in the analysis has a comparatively short ORF2 and no predicted readthrough. c Comparison of amino acid sequences resulting from plausible translation of the ORF2. Percentage of selected amino acids in ORF2 of selected dipterans. The composition did only differ in A. gambiae (p = 0.046) when compared to D. melanogaster. cov., sequence coverage; id. amino acid identity; AAs, number of amino acids in ORF2; kDa, size of ORF2. d MView Sequence alignment of ORF2 in selected dipterans. Left panel, start of ORF2; right panel, C-terminal end of ORF2. e Disorder analysis of Drosophila melanogaster Dfr-L. The intrinsic disorder of Dfr-L was calculated by the VSL2 algorithm (http://www.pondr.com/). Schematic representation of ORF1 (red), ORF2 (yellow), and the DNA-binding domains (DBD, blue) are shown above the disorder graph. The horizontal bold line indicates 0.5 disorder score, above which the amino acid sequence is considered disordered