Fig. 3

The relative frequency of dfr stop codon readthrough varies between tissues and development stages. a–i Confocal images of immunostaining, using the Dfr-S/L antibody recognizing both forms of Dfr (a–c), or the Dfr-L antibody specific for the C-terminal extension (d–i) of larval BRGCs (a, c, and g), larval trachea (b and e), adult male ejaculatory duct (c and f), adult fat body cells (h), and oenocytes (i). Scale bars represent 50 μm. j–l Immunoblot experiments using the Dfr-S/L antibody. Total protein was extracted from BRGCs of female and male wandering L3 larvae (j) or BRGCs of synchronized L3 larvae (k), at indicated time points (hours) ALH. For comparison, 88 h ALH is approximately equal to 40 h ALE in Fig. 6e. j, k Representative blots. Actin was used as loading control. l Quantification of the Dfr-S*:Dfr-L* ratio from (k). The relative concentration of Dfr-L* decreased from early L3 to late L3. The underlying line plot represent means; error bars depict SD (n = 4). Distinct letters above the points represent statistically significant difference (p < 0.05)