Skip to main content
Fig. 5 | BMC Biology

Fig. 5

From: Stop codon readthrough alters the activity of a POU/Oct transcription factor during Drosophila development

Fig. 5

Absence of Dfr-L causes extensive transcriptional changes in the BRGC of wandering larvae. a–h RNA-seq analysis of BRGCs or bodies derived from control versus dfr14. Data were generated from all transcripts with a designated FBgn number and expressed in at least one of the groups in respective tissue. Each tissue was analyzed separately. a, b Two-dimensional scatterplot depicting leading log2 expression differences between the transcriptomes of controls versus dfr14 derived from either BRGC (a) or body (b). c, d Venn diagrams of unaltered, increased or decreased transcript levels in dfr14 (FDR < 0.05) in BRGC (c) and body (d). For full list of DEGs, see Additional File 5. e, f Vulcano plots of all genes in BRGC (e) and body (f), expressed in at least two out of the four groups. Differentially expressed transcripts are highlighted in red. g Gene ontology (GO) analysis was performed using GOrilla (terms with an FDR < 0.1 were considered) and summarized in REVIGO to remove redundant terms (dispensability > 0.5). Separate analyses were performed using either all (top panels), downregulated (middle panels), or upregulated (bottom panels) hits from respective tissue. Circle sizes represent GO term frequency (in log10-scale) in the underlying database, e.g., a small circle depicts a more specific term. Circle color and scale bar reflects log10 FDR value. Scatterplot axes refer to semantic similarities between GO terms within a two-dimensional space (the values have no intrinsic meaning per se). For ease of viewing, the dispensability threshold was set to < 0.2 for spelled out GO terms. For the complete list of GO terms, see Additional file 6. h Comparative bar graph of BRGC expression of Ecdysone biosynthesis genes and dfr. Asterisks indicate differential expression between groups (FDR < 0.01). shade (shd) was included as a negative control since it is not normally expressed in the BRGC. For the complete list of ecdysone-associated genes, see Additional file 7

Back to article page