Skip to main content
Fig. 6 | BMC Biology

Fig. 6

From: Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology

Fig. 6

Validation of mutants identified by REMI-seq. A Mutants identified by REMI-seq exhibit growth advantages or disadvantages. Independent isolates of 19 mutants identified by REMI-seq were tested for growth effects in competition with the parental AX4 strain. 18 mutants showed significant differences, and all mutants identified by REMI-seq as disadvantaged grew more slowly than wild type. B REMI-seq can quantitatively predict fitness effects in growth competition. There is a strong correlation between the experimentally measured growth rate of each mutant and the relative change in abundance of each mutant inferred by REMI-seq at the final round of selection. Similar results can be seen when the data from every round is compared to the growth rate (see Additional file 10)

Back to article page