Fig. 3

Depletion of SRCAP affects cell division in HeLa cells. RNAi knockdown was performed by transfecting HeLa cells with specific siRNAs (see the “Methods” section). Cells were stained with DAPI (blue) and anti-α-Tubulin (green). Left panels (mock), right panels (RNAi). Scale bar = 10 μm. Six classes of defects were categorized: A Histograms showing the quantitative analysis of cell division defects; mocks (white histograms), scramble (light gray histograms), SRCAP A (dark gray histograms), and SRCAP B (black histograms). B Multipolar spindles (MS). C Chromosome misalignments (CM) and abnormal spindle morphology (ASM). D Chromatin bridges (CB). E Long intercellular bridges (LIB); no DAPI-stained trapped chromatin was observed. F Multinucleated cells (MC). G Intercellular distance. The quantitative analysis of defects scored in RNAi-treated and control cells (Table 1) is based on the following numbers: at least 100 prometaphases and metaphases for MS, 70 metaphases for CM and ASM, 300 telophases for LIB and CB, and 5500 for MS. Three independent experiments were performed. *P < 0.05; **P < 0.005; and ***P < 0.0005 compared with the controls group (mock and scramble) by Fisher’s exact test