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Fig. 4 | BMC Biology

Fig. 4

From: The ATPase SRCAP is associated with the mitotic apparatus, uncovering novel molecular aspects of Floating-Harbor syndrome

Fig. 4

Abnormal microtubules re-polymerization in SRCAP depleted HeLa cells. From top to bottom: DAPI (blue), EGFP::α-Tubulin (green) and merge. Hela cells were incubated in ice (1 h) to stimulate microtubules depolymerization (T0). Compared to the non-treated samples (NT), microtubule re-polymerization after 5 min (T5) of rewarming give rise to properly shaped asters in control metaphases (mock), while in SRCAP depleted metaphases (RNAi), aster reformation is clearly aberrant with EGFP::α-Tubulin fluorescence marking only one pole spot, together with sparse and disorganized fibers. The results are based on a total of three experiments; at least 300 cells were scored from both RNAi-treated and control cells. Scale bar = 10 μm

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BMC Biology

ISSN: 1741-7007

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