Fig. 5

SRCAP depletion affects midbody localization of cytokinesis regulators. A Examples of cytokinesis regulators recruitment at midbody in mock and SRCAP depleted HeLa cells (RNAi). From left to the right: DAPI (blue), anti-α-Tubulin (green), cytokinesis regulators (red) and merge. B Histograms showing the quantitative analysis of mis-localizations (see also Table 2); mock (white histograms), SRCAP depleted cells (black histograms). Scale bar = 10 μm. Three independent experiments were performed and at least 300 telophases were scored in both RNAi-treated and control cells. *P < 0.05; **P < 0.005; and ***P < 0.0005 compared with the mock group by Fisher’s exact test