Fig. 7

Bend probability in larval locomotion in the 11 Drosophila species at 32 °C. A Bend probability of the 11 species at 24 °C and 32 °C. The data at 24 °C were the same as in Fig. 3C. Sample numbers at 32 °C were as follows: Dvir: n = 25; Dmel: n = 20; Dmoj: n = 29; Dper: n = 27; Dpse: n = 25; Dsec: n = 20; Dwil: n = 21; Dere: n = 24; Dmau: n = 17; Dana: n = 23; and Dyak: n = 19. p values presented the results of the Mann-Whitney U test. B Scatter plot of bend probability at 32 °C against the minimum habitat temperature, Tmin. C Scatter plot of the difference in bend probability between 32 and at 24 °C (bend probability at 32 °C − bend probability at 24 °C) against the minimum habitat temperature Tmin. In B and C, the median ± sem was shown. The red lines showed the linear regression functions, and the shaded areas represented the 95% confidence bands. The point estimates of the Pearson correlation and their 95% confidence intervals were − 0.850 and [− 0.96, − 0.51] in B and 0.40 and [− 0.26, 0.81] in C, respectively. D–F Examples of trajectories (left) and the bend angle (right) at 24 °C (top) and 32 °C (bottom) of larval locomotion of each species (D Dsec, E Dmel, and F Dwil)