Fig. 3

887S and 887L antagonistically regulate the expression and activity of CA9. A The top 15 upregulated or downregulated genes identified by RNA-seq in the two indicated 887S knockout cell lines (HREmut1 and HREmut2) and the corresponding control lines under hypoxic condition. B The top 15 upregulated or downregulated genes identified by RNA-seq in the two indicated 887L knockdown cell lines (sh887L1 and sh887L2) and the corresponding control lines under normoxic condition. C, D Relative expression levels of the indicated CA family genes and 887S/887L adjacent genes (ATP13A3, CPN2, HES1, LRRC, OPA1) in 887S knockout cells under hypoxic condition (C) or 887L knockdown cells under normoxic condition (D) (n=3). E The protein levels of CA9 in 887S or 887L overexpression cells (n=3). Related to Figure S5E, F. F, G Images of the fluorescence intensity detected by BCECF-AM assay in the indicated 887S or 887L overexpression cells in the presence of normoxia (F) and hypoxia (G). H Extracellular pH values of the indicated control (Ctrl) and 887S knockout cells under hypoxic condition. I Extracellular pH values of the indicated control (Ctrl) and 887L knockdown cells under hypoxic condition. Data are shown as means ± SEMs. P values are calculated using Student’s t test