Fig. 4

887S and 887L drive TSCC progression in opposite directions through CA9 under hypoxia. A Representative images of colony formation assay in 887S knockout cells in the presence of control or 887S overexpressed plasmid. B Statistical analysis according to A (n=3). Related to Figure S5G. C Representative images of colony formation assay in 887L knockdown cells in the presence of control or 887L overexpressed plasmid. D Statistical analysis according to C (n=3). Related to Figure S5H. E Representative images of transwell assay in 887S knockout cells in the presence of control or 887S overexpressed plasmid. F Statistical analysis according to E (n=3). Related to Figure S5G. G Representative images of transwell assay in 887L knockdown cells in the presence of control or 887L overexpressed plasmid. H Statistical analysis according to G (n=3). Related to Figure S5H. I, J Representative images of xenografts from Balb/c (nu/nu) mice injected with 887S knockout (I) or 887L overexpressed (J) TSCC15 cells. The arrows denote tumors in situ. K HE staining image of tumor specimen dissected from the xenograft mice. The enlarged regions indicate the keratinized feature of TSCC15. L Representative images of colony formation assay in 887S overexpressed cells in the presence of control or CA9-overexpressed plasmid. M Statistical analysis according to L (n=3). Related to Figure S7B. N Representative images of colony formation assay in 887L overexpressed cells in the presence of control or siCA9. O Statistical analysis according to N (n=3). Related to Figure S7C. P Representative images of transwell assay in 887S knockout cells in the presence of control or siCA9. Q Statistical analysis according to P (n=3). R Representative images of transwell assay in 887L knockdown cells in the presence of control or CA9-overexpressed plasmid. S Statistical analysis according to R (n=3). Data are shown as means ± SEMs. P values are calculated using Student’s t test