Fig. 5

887L is required for the HIF1α-induced activation of CA9 under hypoxia. A HIF1α ChIP assay on the indicated HRE site and control region on CA9 promoter upon normoxia and hypoxia (n=3). B Relative expression levels of CA9 under normoxia and hypoxia (n=3). C Activity changes of CA9 promoter in the 887L knockdown cells or 887S knockout cells (n=3). D Activity change of CA9 promoter in the 887L overexpressed cells or 887S overexpressed cells (n=3). E HIF1α RIP assay showed the immunoprecipitation of the indicated β-actin (ACTB), 887S, and 887L RNAs upon normoxia and hypoxia (n=3). F Capture of 887L RNA by RNA pull-down assay in 293T cells, that do not express 887L, and TSCC15 cells. NC: non-probe control; even and odd: two separated pools of 887L probes containing either even or odd numbered probes based on their positions along the 887L sequence. 887L-P1, 887L-P2, and 887L-P3: primers for 887L detection; GAPDH: primers for GAPDH detection. (n=2). G ChIRP assay showed the association of the 887L RNA with the CA9’s HRE site under normoxia and hypoxia (n=2). H Recruitment of HIF1α to the control region and HRE site of CA9 promoter in 887S knockout and 887L knockdown cells (n=3). Data are shown as means ± SEMs. P values are calculated using Student’s t test