Fig. 4.

Detailed morphology of cerebral organs in juveniles of L. ruber. TEM micrographs of cerebral organs in 60-day-old juvenile, showing cross-section (A) and details of particular regions of the organ (D–G). Z-projections of cerebral organs in 42-day-old juveniles visualized with Sytox green nuclear staining and in situ hybridization with probe against ChAT (cyan and red, respectively; B) and antibodies against FMRF-amide and tyrosinated tubulin (magenta and yellow, respectively; C). Cerebral organs are outlined in red (A) and white (B, C). Orientation inside the animal is indicated in the top-right corners in A–C (A, anterior; P, posterior; D, dorsal; V, ventral; M, median; L, lateral). Micrographs in D–G show magnified areas of A. White outlined boxes on E, F, and G indicate areas magnified in corresponding insets. ax, neuroglia axon; bc1, bipolar cell type1; bc2, bipolar cell type 2; bv, blood vessel; cc, ciliated canal; ccc, ciliated canal cell; con, cerebral organ nerve; cr, ciliary rootlet; dbl, dorsal brain lobe; ds, desmosome; ga, Golgi apparatus; gc, ganglion cell; lcc, dilated cilia of lappet cell; lnc, longitudinal nerve cord; mjc, major ciliated canal; mnc, minor ciliated canal; mt, mitochondrium; ng, neuroglia; ngc, neuroglandular cell; pb, proboscis; ry, rhynchocoel. White arrow indicates ChAT⁺ cells in cerebral organ, and white arrowhead FMRF-amide-like immunoreactivity in cerebral organ