Skip to main content
Fig. 4. | BMC Biology

Fig. 4.

From: Molecular and morphological analysis of the developing nemertean brain indicates convergent evolution of complex brains in Spiralia

Fig. 4.

Detailed morphology of cerebral organs in juveniles of L. ruber. TEM micrographs of cerebral organs in 60-day-old juvenile, showing cross-section (A) and details of particular regions of the organ (DG). Z-projections of cerebral organs in 42-day-old juveniles visualized with Sytox green nuclear staining and in situ hybridization with probe against ChAT (cyan and red, respectively; B) and antibodies against FMRF-amide and tyrosinated tubulin (magenta and yellow, respectively; C). Cerebral organs are outlined in red (A) and white (B, C). Orientation inside the animal is indicated in the top-right corners in AC (A, anterior; P, posterior; D, dorsal; V, ventral; M, median; L, lateral). Micrographs in DG show magnified areas of A. White outlined boxes on E, F, and G indicate areas magnified in corresponding insets. ax, neuroglia axon; bc1, bipolar cell type1; bc2, bipolar cell type 2; bv, blood vessel; cc, ciliated canal; ccc, ciliated canal cell; con, cerebral organ nerve; cr, ciliary rootlet; dbl, dorsal brain lobe; ds, desmosome; ga, Golgi apparatus; gc, ganglion cell; lcc, dilated cilia of lappet cell; lnc, longitudinal nerve cord; mjc, major ciliated canal; mnc, minor ciliated canal; mt, mitochondrium; ng, neuroglia; ngc, neuroglandular cell; pb, proboscis; ry, rhynchocoel. White arrow indicates ChAT+ cells in cerebral organ, and white arrowhead FMRF-amide-like immunoreactivity in cerebral organ

Back to article page