Fig. 5.

Expression of investigated transcription factors in the heads of 42-day-old juveniles of L. ruber. A–X Fluorescent in situ RNA hybridization, for each panel the name of the hybridized gene is shown in the white box above the micrographs. Fluorescent signal from RNA probes is in red, from antibody staining against tyrosinated tubulin in yellow and brain lobes are outlined in white. All animals are shown in dorso-ventral projection with anterior to the top; the letter in the top-right corner of each panel indicates whether focus is on dorsal (d) or ventral (v) structures. Detailed expression patterns are described in the text. Magenta arrowheads indicate expression in the cerebral organs, blue in the rhynchocoel, green in the lateral cephalic slits, and white in the proboscis. Scale bars 20 μm. Y Map of gene expression in the L. ruber brain. Gray bars indicate that gene is expressed in a particular brain region. DA, dorso-anterior brain domain; DL, dorso-lateral brain domain; DM, dorso-median brain domain; VA, ventro-anterior brain domain; VL, ventro-lateral brain domain; VM, ventro-median brain domain