Fig. 6.

Details of gene expression in the cerebral organs of 42-day-old juveniles of L. ruber. A Schematic drawing of the cerebral organ and accompanying neural structures, orientation in the animal is indicated in bottom-right corner (A, anterior; P, posterior; M, median; L, lateral). cc, ciliated canal; con, cerebral organ nerve; dbl, dorsal brain lobe. B–J Fluorescent in situ RNA hybridization; for each panel, the name of hybridized gene is provided in the bottom-right corner. Fluorescent signal from RNA probes is in red, from antibody staining against tyrosinated tubulin in yellow, and from Sytox green nuclear staining in cyan; cerebral organs are outlined in white. The detailed expression patterns are described in the text. White arrowhead indicates arx⁺ cell at the posterior side of the ciliated canal opening. Scale bars 10 μm