Skip to main content
Fig. 5 | BMC Biology

Fig. 5

From: Profiling of chromatin accessibility identifies transcription factor binding sites across the genome of Aspergillus species

Fig. 5

Identification of de novo predicted footprints in A. niger CBS513.88 and SH2, and functional verification in regulating gene expression. a Venn diagram analysis of identified footprints in A. niger CBS513.88 and SH2. b Statistical analysis of the distances between the identified DNA footprints and the TSSs. c De novo prediction of transcription factors in A. niger CBS513.88 (red) and SH2 (green). The circle size represents the enrichment fold. The top three transcription factor motifs in A. niger SH2 are labeled with the corresponding binding motifs. The x-axis represents P value. d In vivo functional verification of putative de novo transcription factor targeting sites were carried out by artificially synthesized minimal promoters. The red dividing line represents the location of the footprints to be verified. The red text on the side is the TF binding motif contained in the verified DNA instances. From top to bottom, there were 2 E-box of bHLH factors (motif TCACGTGATC), 3 core elements of bZIP factors and 3 SltA homologs binding motif (motif CTGCCTGAGGCA) target sites validated by phenotype plates in vivo

Back to article page