Fig. 12

Pgt 21-0 RNAi and 5mC methyltransferase gene expression. A Hierarchical clustering of expression levels of Pgt RNAi genes in transcripts per million (logTPM, red colour intensity relates to high expression). The Pgt RNAi RdRPs 1/3, argonaute 1 and dicer 3 show distinct high expression at sporulation in the later stages of infection (6–7 dpi). The 5mC methyltransferases DNMT1 and DNMT5 are expressed across all conditions. B The Pgt argonaute proteins have diversified on the sequence level and AGO 1 and AGO 2 show differences in protein domains. C A phylogenetic tree of Arabidopsis argonaute proteins (At_AGO1-10) and other rust argonautes (Mlp, Melampsora larici-populina; Ml, Melampsora lini; Pt, Puccinia triticina; Pst, Puccinia striiformis f. sp. tritici; PGT, Puccinia graminis p7a) supports the diversification of cereal rust argonautes into two classes, AGO1 and AGO2