Fig. 7

Pgt sRNA differential expression analysis. A A multi-dimensional scaling plot using the edgeR package shows the clustering of the replicates for the different samples. The 3 dpi and 5 dpi samples show less differences in expression than the germinated spores and 7 dpi samples. B Venn diagrams of up-regulated Pgt sRNAs shared between the different time points: germinated spores, early infection (3 dpi and 5 dpi) and late infection (7 dpi). Two major classes of sRNAs occur: one that is up-regulated during late infection (n = 3046) and one that is up-regulated in germinated spores compared to late infection (n = 2315). C Sequence lengths and D 5′ nucleotide distribution of the Pgt sRNAs. Pgt sRNAs up-regulated during late infection differ in length distribution and 5′ nucleotide preference to the remaining Pgt sRNAs. 22-nt Pgt sRNAs up-regulated during late infection strongly prefer a 5′ adenine, which is not observed for 22-nt sRNAs expressed in the other conditions