Fig. 8

Segregation of mitosomes occurs also when mitosis is impaired. The inhibition of the microtubule assembly results into the formation of aberrant cells with impaired nuclear segregation. A Vast majority of cells in untreated cell population are in the interphase stage containing two nuclei (N) and one flagellar apparatus (M) (2N1M). Less than 1% of cells undergo mitosis that ends with a cell carrying 4 nuclei and 2 flagellar apparatuses (4N2M). B Four cell types can be detected in the cell population classified according to the number of the nuclei and the flagellar apparatuses. The cell type 2N2M is pre-cytokinesis; the other cell types represent cells which underwent cytokinesis. Examples of the aberrant morphologies are shown. Immunolabeling by anti-β-tubulin antibody (green), mitosomes by anti-GL50803_9296 antibody (red). Nuclei are stained with DAPI (blue). The central mitosomes highlighted by white arrowheads. C The quantification of morphologies in albendazole treated (n = 334 cells) and untreated (n = 350 cells) populations