Fig. 2.

Genome size variation is linked to sequences with elevated GC-content. a GC-distribution of all short-read libraries combined (29 libraries of 15 rotifer clones). The red, cyan, and blue lines designate a mixture model fitted to these data, consisting of three normally distributed subpopulations (26 ± 6, 36 ± 2.5, and 48 ± 3 %GC; means and SDs). b Panels show the results of the same mixture model applied to each library individually, thus estimating the proportion of the total reads per library in each GC-fraction. 2C-genome size estimates are based on flow cytometry and were taken from [7]. Colors in b correspond to the six library preps (A–F) listed in Table S4: A = orange, B = gold, C = green, D = turquoise, E = blue, and F = pink