Fig. 4.

Genomic regions of elevated coverage variability are responsible for genome size variation. The data in this figure are based on normalized coverage (calculated for 50-kbp windows) of 29 short-read libraries from 15 rotifer clones. a Genomic regions differ in coverage variability (measured as the standard deviation of coverage among libraries) as there are regions of low, intermediate, and high variability. b, c Mean coverage in regions of elevated coverage variation (interSD, highSD) correlates positively with genome size (flow cytometry data on genome size was taken from [7]). Dots in c represent the mean coverage per library. Colors indicate co-prepared libraries (of which some were prepared from the same rotifer clone, but at different dates and with different library preparation methods; see Table S4). Colors in c correspond to the six library preps (A–F) listed in Table S4: A = orange, B = gold, C = green, D = turquoise, E = blue, and F = pink. d This panel shows the same data as in c, but rotifer clones are categorized into small 2C genome sizes (<430Mbp, which represent the basal genome size of B. asplanchnoidis according to [7]) vs. large genome sizes (>430Mbp, which contain additional genomic elements that independently segregate during meiosis, according to [7])