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Fig. 6 | BMC Biology

Fig. 6

From: Construction of à la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins

Fig. 6

A model for the creation of an ALACAT resource. From a library of Qbricks (that may include redundant sequences to increase the choice of quantotypic peptides for specific proteins) assembly into short ALACATs would provide a test system to assess the suitability of peptides for quantification. Once the short ALACATs are optimised, sets of them could subsequently be assembled into long ALACATs. Long ALACAT DNA can be readily used to drive protein synthesis in the end user laboratory, labelled as appropriate

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